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KMID : 0613820100200101563
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Journal of Life Science
2010 Volume.20 No. 10 p.1563 ~ p.1568
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Increase in the Chlorophyll Contents by Over-expression of GmNAP1 Gene in Arabidopsis Plant
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Park Phun-Bum
Ahn Chul-Hyun
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Abstract
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In the course of a research concerning the molecular mechanism of hypocotyl elongation that occurs during soybean seedling growth in darkness, we have generated a number of ESTs from a cDNA library prepared from the hypocotyls of dark-grown soybean seedlings. Comparison of the ESTs assigned a cDNA clone as a putative plastidic ATP-binding-cassette (ABC) protein homologue. The soybean GmNAP1 protein contains an N-terminal transit peptide which targets it into the chloroplast. The transcription level of the GmNAP1 gene was investigated under continuous red light, continuous far-red light, and complete darkness. The main function of this NAP1 protein is the transport of protoporphyrin IX which is the precursor of chlorophyll from the cytoplasm to the chloroplast. The GmNAP1 gene was transferred into the Arabidopsis under the CaMV 35S promoter. The chlorophyll level of this transgenic Arabidopsis plant was much higher than the chlorophyll level of the wild type Arabidopsis plant.
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KEYWORD
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Nonintrinsic ABC protein, chlorophyll, protoporphyrin X, soybean
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